Refine your search
Collections
Co-Authors
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Chuku, L. C.
- Analysis of Hematological and Biochemical Parameters, to Study the Ecological Relationship on Selected Species of Vertebrates
Abstract Views :515 |
PDF Views:342
Authors
L. C. Chuku
1,
A. A. Uwakwe
1
Affiliations
1 Department of Biochemistry, University of Port Harcourt, Rivers State, NG
1 Department of Biochemistry, University of Port Harcourt, Rivers State, NG
Source
Indian Journal of Drugs and Diseases, Vol 1, No 9 (2012), Pagination: 208-212Abstract
Objectives: Hematological and biochemical analysis studies of ruminant vertebrates (cow, sheep and goat) will provide us an acceptable method for understanding the ecological relationship among the vertebrates. Methods: Haematological/Biochemical studies were carried out on blood from the ruminants: Goat (Capra hircus-L), sheep (Ovis Aries dolrchra), cattle (Bos taurus) and human (Homosapiens) as control. Results: Electrophoretically, goat blood revealed three different Hb polymorphic types: HbAA (45%), HbAS (50%) and HbSS (5%). Only the 'O' Rh blood group was observed in all cases, and GST values of 4.05-6.30 I.U were obtained on the other hand. Cow Hb electrophoresis produced two patterns corresponding to the human HbAA and HbAS types. Only the 'O' Rh- blood group was obtained in all cases and GST values of 2.85-5.02 I.U were obtained, while sheep blood produced only the HbAA-type, only the 'O' blood group was identified in all case. GST activity values were in the range of 3.05-4.52 IU. Conclusion: These results could point to a definite haematological and biochemical similarity between man and ruminants and thus serves as a springboard for further genetic studies using lower vertebrate's as research models.Keywords
Haemoglobin, Glutathione-S-transferase, Genotype, ErythrocyteFull Text
References
- Anosike EO, Uwakwe AA, Monanu MO and Ekeke GI (1991) Studies on Human Erythrocyte Glutathione-S-Transferase from HbAA, and HbSS subjects. Biochem. Biomed. 50, 1051-1055.
- Best and Taylor (1982) Physiological Basis of Medical Practice, 9th Edn. NY Press. pp- 500-587.
- Boyland E and Chasseaud LF (1969) The role of Glutatione and Glutathione-S-Transferase in mercapturic acid synthesis. Adv. Enzymol. 32, 173-219.
- Chasseaud LF (1979) The role Of Glutathione And Glutathione-S-Transferases in the Metabolism Of Chemical Carcinogens and other electrophilic agents. Adv. Cancer Resour. 29, 175-177.
- Goodwin BL (1967) Handbook of intermediary metabolism of chromatic compounds. John Wiley and Sons. Inc. NY. pp:85-87.
- Harvey JW and Bewtler E (1982) Binding of heme by Glutathione-S-Transferase. A possible role of the Erythrocyte Enzyme Blood. 60, 1227-1230.
- Holfbrand AV and Lewis SM (1989) Postgraduate Haematology. Heinemann Professional Publishing London. pp: 121-132.
- Jacoby WB (1978) Glutathione-S-Transfease. A group of multi-functional detoxification proteins. In advances in Enzymology. Meiseter A edn. John-Willey Inter-Science, NY. pp : 383-414.
- Lawrence EG (2000) Vertebrate. In the World Book Encyclopedia- World Book Inc. Midigan. pp: 340.
- Lehninger AL (1982) Principle of Biochemistry. North Publisher, Inc.USA. pp : 706-711.
- Passmore R and Robson JS (1983): Pharmacology, Microbiology, General Pathology and Related Subjects. Blackwell Scientific Publication, Oxford, London, 2nd Edn. pp: 332-684.
- Pauling L, Itano HA, Singer SJ and Wells IC (1949) Sickle cell disease, a Molecular disease. Sci. 110, 543-548.
- Walter JR (1997) Vertebrate. In Encyclopedia of Science andTechnology, McGraw-Hill. NY. pp :225-226.
- Haematological and Biochemical Studies on Selected Species of Fishes
Abstract Views :339 |
PDF Views:271
Authors
L. C. Chuku
1,
A. A. Uwakwe
1
Affiliations
1 Department of Biochemistry, University of Port Harcourt, Rivers State, NG
1 Department of Biochemistry, University of Port Harcourt, Rivers State, NG
Source
Indian Journal of Drugs and Diseases, Vol 1, No 8 (2012), Pagination: 185-191Abstract
In this study the following biochemical and haematological studies on Catfish (Clarius gariepianus); Mullet (Mugi cephalus), Tilpia (Oreochromis niloticus), mudskipper (Gobi I), African sleeper (Gobi-II), were carried out; Haemmoglobin electrophorosis, ABO Blood grouping and Glutathine-S-Transferase (GST) activity. About 50% of catfish indicated the Rh.D- while thew remaining 50% indicated the Rh.D+ blood group/. The 'A' 'O' and 'AB' blood groups were detected and glutathione-S- transferase activity values of 5.64 - 7.27 I.U were obtained. Hb concentration values were of the range of 5.5 -10.7gm% blood group results gave values of 50% D+ (A,O) and 50% (AB). GST activity values of 13.50-20.10 I.U were obtained. Gobi-I and Gobi-II Hb electrophoretic pattern was similar to human HbSS blood with Hb concentration range of 1.8-5.57gm%. GST activity values of 16.97-33.08 I.U. were obtained. Haemoglobin electrophoresis of Tilapia produced multiple patterns corresponding to human HbSS, HbCC and possibly HbCD. Hb concentration values were of the range of 3.70-12.94gm% and GST opf 9.90-12.92 I.U were obtained. Only the 'O' and 'AB' blood groups were identified.Keywords
Haemoglobin, Glutathione-S-Transferase, Genotype, SerumFull Text
References
- Anosike EO, Uwakwe AA, Monanu MO, Ekeke GI (1991) Studies on Human erythrocyte Gluthathine-S-Transferase from HbAA, HbAS and HbSS subjects. Biochem. Biomed. Acta. 50, 1051-1055.
- Christensen MG, Fiandt JJ and Poeschil AB (1978) Cells properties of Bischolar_main front. J. Fish Biol. 12, 51-60.
- Harris JW and Kellermeyer RW (1974) The Red Cell (Harris JW and Kellermeyer RW Edn) 3rd Edn. Harvard University Press,- Cambridge, Massachusetts. pp: 517-527.
- Ingram VM (1956) A specific chemical difference between the Globins of normal human and sickle cell Haemoglobin. Nature 178: 792-797.
- Kirpinichnikov VS (1981) Genetic basis of fish selection. Springer-Verley (NY), pp: 45-50.
- Martin DW, Jnr, Mayers PA and Rodwell WW (1983) Harpers review of Biochemistry. Lange Medical Publications, California. 30-40, 400-500.
- Pauling L, Itano HA, Singer SJ and Wells IC (1949): Sickle Cell Disease: A molecular disease. Sciences 110, 543-548.
- Sick K (1961) Haemoglobin polymorphism in fish. Nature (London) 192: 894-896.
- Stanburg BJ, Wyngarder BJ and Dowal DSF (1978) The Metabolic basis of inherited diseses. McGraw-Hill Book Company, NY, 1474-1501.
- Uwakwe AA and Anosike EO (1992) Electrophoretic techniques in Field Herbarium and Lab. Techniques. (B.E. Okoh Editor). Mebeyi Associates (Publ) Port Harcourt, Nigeria.
- Walter JR (1997) Vertebrate in encyclopedia of science and technology McGraw-Hill, NY. pp: 225-226.
- World Book Encyclopedia (2000): World Book Inc. pp: 340-341.
- Assessment of Polycyclic Aromatic Hydrocarbon Levels in Blood of Refinery Workers in Nigeria
Abstract Views :160 |
PDF Views:0
Petrol (gasoline) is composed of aromatic and polycyclic hydrocarbons which release carcinogenic and dangerous refinery fumes, when combusted in plants and thus health hazards. Levels of polycyclic aromatic hydrocarbon (PAH) in the serum of refinery workers (men) in Oil zone plants in Nigeria were therefore analyzed.
Purpose of the study
The study intended to find out the levels of PAH in Oil zone plant workers, with 10-27 years of exposure. This will enable us to know the exposure rate and advice the workers appropriately.
Method
Gas chromatography equipped with flame ionization detector (FID) was used. The men had worked for 8 hrs per day and 5 days per week, for 10-13, 14-19 and 20-27 years for groups A, B and C respectively. The Control group (D) comprised of 10 office workers (non-smokers) who had worked for 10-27 years.
Results
The levels of PAH (with only acenaphthene and acenaphthylene) in gasoline sample were 66.74701±0.03355 ppm/ml. PAH in the blood of workers in groups A-D were below detection limit (BDL).
Conclusion
The nature and the levels of PAH and TPH found in gasoline sample and blood of the refinery workers seem not to be deleterious to health but continuous exposure of humans to these hydrocarbons may have a long term effect.
Authors
Affiliations
1 Department of Biochemistry, University of Port Harcourt, Choba, Rivers State, NG
1 Department of Biochemistry, University of Port Harcourt, Choba, Rivers State, NG
Source
Asian Journal of Research in Chemistry, Vol 3, No 3 (2010), Pagination: 801-804Abstract
BackgroundPetrol (gasoline) is composed of aromatic and polycyclic hydrocarbons which release carcinogenic and dangerous refinery fumes, when combusted in plants and thus health hazards. Levels of polycyclic aromatic hydrocarbon (PAH) in the serum of refinery workers (men) in Oil zone plants in Nigeria were therefore analyzed.
Purpose of the study
The study intended to find out the levels of PAH in Oil zone plant workers, with 10-27 years of exposure. This will enable us to know the exposure rate and advice the workers appropriately.
Method
Gas chromatography equipped with flame ionization detector (FID) was used. The men had worked for 8 hrs per day and 5 days per week, for 10-13, 14-19 and 20-27 years for groups A, B and C respectively. The Control group (D) comprised of 10 office workers (non-smokers) who had worked for 10-27 years.
Results
The levels of PAH (with only acenaphthene and acenaphthylene) in gasoline sample were 66.74701±0.03355 ppm/ml. PAH in the blood of workers in groups A-D were below detection limit (BDL).
Conclusion
The nature and the levels of PAH and TPH found in gasoline sample and blood of the refinery workers seem not to be deleterious to health but continuous exposure of humans to these hydrocarbons may have a long term effect.
